Pengaruh Ekstrak Daun Buncis (Phaseolus vulgaris L.) Terhadap Jumlah Fibroblast Kulit dalam Penyembuhan Luka Bakar

Putri Satria Ningsih, Muhammad Arafat Muchtar, Riri Virzan Putri

Abstract


Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun buncis (Phaseolus vulgaris L.) terhadap peningkatan jumlah fibroblas pada kulit selama proses penyembuhan luka bakar. Penelitian ini menggunakan model tikus putih jantan galur Wistar yang diinduksi luka bakar. Tikus dibagi menjadi beberapa kelompok perlakuan, yaitu kelompok kontrol negatif, kelompok kontrol positif, dan kelompok perlakuan dengan berbagai dosis ekstrak daun buncis. Ekstrak dioleskan secara topikal selama periode tertentu. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun buncis secara signifikan meningkatkan jumlah fibroblas dibandingkan dengan kelompok kontrol negatif. Hal ini menunjukkan potensi ekstrak daun buncis sebagai agen penyembuhan luka bakar melalui mekanisme stimulasi proliferasi fibroblas. Penelitian ini memberikan kontribusi yang signifikan terhadap pengembangan terapi alami untuk penyembuhan luka bakar.


Keywords


Ekstrak Daun Buncis; Fibroblas; Luka Bakar; Penyembuhan Luka; Tikus Wistar.

Full Text:

PDF

References


Bai, Q., Li, M., Zhou, J., Imran, A., de Souza, T. S. P., Barrow, C., Dunshea, F., & Suleria, H. A. R. (2024). Influence of Processing Methods on Phytochemical Composition of Different Varieties of Beans (Phaseolus vulgaris). Food Reviews International, 40(7), 1941–1979. https://doi.org/10.1080/87559129.2023.2245026

Bodas, K., & Shinde, V. (2021). Healing of Wounds: A Detailed Review on Models, Biomarkers, Biochemical and Other Wound Assessment Parameters. International Journal of All Research Education and Scientific Methods (IJARESM), 9(3), 2455–6211.

Boukhatem, M. N., Ferhat, M. A., Kameli, A., Saidi, F., & Kebir, H. T. (2014). Lemon grass (cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. Libyan Journal of Medicine, 9, 1–10. https://doi.org/10.3402/ljm.v9.25431

Brunicardi, F. C., Andersen, D. K., Billiar, T. R., Dunn, D. L., Hunter, J. G., Matthew, J. B., & Pollock, R. E. (2015). Schwartz’s Principles of Surgery. In Schwartz’s Principles of Surgery (10th ed., pp. 1–2097). McGraw-Hill Education.

Candra, L. M. M., Andayani, Y., & Wirasisya, D. G. (2021). Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus vulgaris L.). Jurnal Pijar Mipa, 16(3), 397–405. https://doi.org/10.29303/jpm.v16i3.2308

Cay, M., Ozbag, D., Ucar, C., Altay, Z., Senol, D., & Yildiz, S. (2017). The Effect of Cortisol Level Increasing Due to Stress in Healthy Young Individuals on Dynamic and Static Balance Scores. Northern Clinics of Istanbul, 5(4), 295–301. https://doi.org/10.14744/nci.2017.42103

Ebrahim, A. E., Abd El-Aziz, N. K., Elariny, E. Y. T., Shindia, A., Osman, A., Hozzein, W. N., Alkhalifah, D. H. M., & El-Hossary, D. (2022). Antibacterial activity of bioactive compounds extracted from red kidney bean (Phaseolus vulgaris L.) seeds against multidrug-resistant Enterobacterales. Frontiers in Microbiology, 13(November). https://doi.org/10.3389/fmicb.2022.1035586

Eivazzadeh-Keihan, R., Moghim Aliabadi, H. A., Radinekiyan, F., Sobhani, M., Farzane Khalili, Maleki, A., Madanchi, H., Mahdavi, M., & Shalan, A. E. (2021). Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin-agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles. RSC Advances, 11(29), 17914–17923. https://doi.org/10.1039/d1ra01300a

Escolano, M., Gaviña, D., Alzuet-Piña, G., Díaz-Oltra, S., Sánchez-Roselló, M., & Pozo, C. del. (2024). Recent Strategies in the Nucleophilic Dearomatization of Pyridines, Quinolines, and Isoquinolines. Chemical Reviews, 124(3), 1122–1246. https://doi.org/10.1021/acs.chemrev.3c00625

Fenwick, D. E., & Oakenfull, D. (1983). Saponin content of food plants and some prepared foods. Journal of the Science of Food and Agriculture, 34(2), 186–191. https://doi.org/10.1002/jsfa.2740340212

Ganesan, K., & Xu, B. (2017). Polyphenol-rich dry common beans (Phaseolus vulgaris L.) and their health benefits. International Journal of Molecular Sciences, 18(11). https://doi.org/10.3390/ijms18112331

Ghozali, I. (2018). Aplikasi Analisis Multivariate dengan Program IBM SPSS 25. In Badan Penerbit Universitas Diponegoro.

Gonzalez, A. C. D. O., Andrade, Z. D. A., Costa, T. F., & Medrado, A. R. A. P. (2016). Wound healing - A literature review. Anais Brasileiros de Dermatologia, 91(5), 614–620. https://doi.org/10.1590/abd1806-4841.20164741

Hrapkiewicz, K., Colby, L., & Denison, P. (2013). Clinical Laboratory Animal Medicine (4th ed.). Wiley Blackwell.

Kendall, L. V., Owiny, J. R., Dohm, E. D., Knapek, K. J., Lee, E. S., Kopanke, J. H., Fink, M., Hansen, S. A., & Ayers, J. D. (2018). Replacement, Refinement, and Reduction in Animal Studies With Biohazardous Agents. ILAR Journal, 59(2), 177–194. https://doi.org/10.1093/ilar/ily021

Kurniawati, A., Harmono, H., & Savira, A. O. (2024). Effect of Purple Leaves Extract on Gingival Collagen Density in Wistar Rats Induced by Porphyromonas Gingivalis. Malaysian Journal of Medicine and Health Sciences, 20(6), 107–113. https://doi.org/10.47836/mjmhs.20.s12.17

Kurniawati, D., Sutrisna, E., & Wahyuni, A. S. (2012). Uji Penurunan Kadar Glukosa Darah Oleh Ekstrak Etanol 70% Daun Buncis (Phaseolus vulgaris L) Pada Kelinci Jantan Yang Dibebani Glukosa. Biomedika, 5(1), 1–8. https://doi.org/10.23917/biomedika.v4i1.257

Lalita, C., & Shalini, G. (2019). Journal of drug delivery and therapeutics (jddt). Journal of Drug Delivery & Therapeutics, 9(3), 661–668. https://doi.org/10.22270/jddt.v10i5-s.4430

Madhujith, T., & Shahidi, F. (2005). Antioxidant Potential of Pea Beans ( Phaseolus vulgaris L .). Journal of Food Science, 70(1).

McLatchie, G., Borley, N., & Chikwe, J. (2013). Oxford Handbook Of Clinical Surgery (4th ed.). Oxford University Press.

Mirhaj, M., Labbaf, S., Tavakoli, M., & Seifalian, A. M. (2022). Emerging treatment strategies in wound care. International Wound Journal, 19(7), 1934–1954. https://doi.org/10.1111/iwj.13786

Nawaz, H., Shad, M. A., Rehman, N., Andaleeb, H., & Ullah, N. (2020). Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Brazilian Journal of Pharmaceutical Sciences, 56. https://doi.org/10.1590/s2175-97902019000417129

Notoatmodjo, S. (2022). Metodologi Penelitian Kesehatan (3rd ed.). Jakarta: Rineka Cipta.

Prasetyono, T. O. H. (2009). General concept of wound healing, revisited. Medical Journal of Indonesia, 18(3), 208–216. https://doi.org/10.13181/mji.v18i3.364

Rehman, K., & Zulfakar, M. H. (2014). Recent advances in gel technologies for topical and transdermal drug delivery. Drug Development and Industrial Pharmacy, 40(4), 433–440. https://doi.org/10.3109/03639045.2013.828219

Reyes-Martínez, A., Almaraz-Abarca, N., Gallardo-Velázquez, T., González-Elizondo, M. D. S., Herrera-Arrieta, Y., Pajarito-Ravelero, A., Alanís-Bañuelos, R. E., & Torres-Morán, M. I. (2014). Evaluation of foliar phenols of 25 Mexican varieties of common bean (Phaseolus vulgaris L.) as antioxidants and varietal markers. Natural Product Research, 28(23), 2158–2162. https://doi.org/10.1080/14786419.2014.930855

Riskesdas. (2019). Laporan Provinsi Sumatera Utara Riskesdas 2018. In Badan Penelitian dan Pengembangan Kesehatan.

Rodrigues, M., Kosaric, N., Bonham, C. A., & Gurtner, G. C. (2019). Wound healing: A cellular perspective. Physiological Reviews, 99(1), 665–706. https://doi.org/10.1152/physrev.00067.2017

Roy, M., Sarker, A., Azad, M. A. K., Shaheb, M. R., & Hoque, M. M. (2020). Evaluation of antioxidant and antimicrobial properties of dark red kidney bean (Phaseolus vulgaris) protein hydrolysates. Journal of Food Measurement and Characterization, 14(1), 303–313. https://doi.org/10.1007/s11694-019-00292-4

Shah, B. N., & Seth, A. K. (2010). Pharmacognosy and Phytochemistry. In Phytochemistry. Reed Elsevier India Private Limited. https://doi.org/10.1016/0031-9422(72)85069-6

Suwarno, B., Ginting, C. N., Girsang, E., & Alamsyah, B. (2025). Pengantar Metodologi Penelitian Kuantitatif, Kualitatif dan Mixed Method (Studi Case Manajemen, Pendidikan, Kesehatan dan Teknik). Saba Jaya Publisher.

Swieringa, F., Spronk, H. M. H., Heemskerk, J. W. M., & van der Meijden, P. E. J. (2018). Integrating platelet and coagulation activation in fibrin clot formation. Research and Practice in Thrombosis and Haemostasis, 2(3), 450–460. https://doi.org/10.1002/rth2.12107

Velnar, T., & Gradisnik, L. (2018). Tissue Augmentation in Wound Healing: the Role of Endothelial and Epithelial Cells. Medical Archives, 72(6), 444–448. https://doi.org/10.5455/medarh.2018.72.444-448

Werner, S., Krieg, T., & Smola, H. (2007). Keratinocyte-fibroblast interactions in wound healing. Journal of Investigative Dermatology, 127(5), 998–1008. https://doi.org/10.1038/sj.jid.5700786

Żwierełło, W., Piorun, K., Skórka-Majewicz, M., Maruszewska, A., Antoniewski, J., & Gutowska, I. (2023). Burns: Classification, Pathophysiology, and Treatment: A Review. International Journal of Molecular Sciences, 24(4). https://doi.org/10.3390/ijms24043749




DOI: https://doi.org/10.51849/j-p3k.v6i2.765

Refbacks

  • There are currently no refbacks.


slot dana

slot